出品公司: | Sigma |
---|---|
载体名称: | pFLAG CMV 5.1 |
质粒类型: | 哺乳动物细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | CMV |
载体大小: | 4682 bp |
5' 测序引物及序列: | M13 fwd |
3' 测序引物及序列: | M13 rev |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | -- |
备注: | 对于短暂的,细胞质表达的C-末端标记融合蛋白。 |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
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pFLAG CMV 5.1载体质粒基本信息
pFLAG CMV 5.1质粒图谱载体图谱和pFLAG CMV 5.1载体序列质粒序列多克隆位点信息
pFLAG CMV 5.1质粒载体简介
pFLAG CMV 5.1质粒序列载体序列
LOCUS pFLAG-CMV-5.1 4682 bp ds-DNA circular SYN 23-OCT-2018 DEFINITION Vector for expression of C-terminally FLAG-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS pFLAG-CMV-5.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4682) AUTHORS MilliporeSigma TITLE Direct Submission JOURNAL Exported Wednesday, March 11, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4682 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 318..697 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 924..990 /note="MCS" /note="multiple cloning site" CDS 993..1016 /codon_start=1 /product="FLAG epitope tag, followed by an enterokinase cleavage site" /note="FLAG" /translation="DYKDDDDK" polyA_signal 1023..1645 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" rep_origin 1854..1989 /note="SV40 ori" /note="SV40 origin of replication" rep_origin complement(2476..3064) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3235..4095) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4096..4200) /gene="bla" /note="AmpR promoter" rep_origin 4227..4682 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attaagcttg cggccgcgaa ttcatcgata gatctgatat 961 cggtaccagt cgactctaga ggatcccggg ctgattacaa ggacgacgat gacaagtagg 1021 ttgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc 1081 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt 1141 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga 1201 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt 1261 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt 1321 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac 1381 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac 1441 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc 1501 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc 1561 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt 1621 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc 1681 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 1741 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1801 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1921 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1981 ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt 2041 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 2101 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 2161 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 2221 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2281 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 2461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 2881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 4201 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 4261 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 4321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 4381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 4441 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 4501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 4561 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 4621 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 4681 tt //
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pFLAG CMV 5.1质粒载体应用举例
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