| 出品公司: | Sigma |
|---|---|
| 载体名称: | pFLAG CMV 5.1 |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 4682 bp |
| 5' 测序引物及序列: | M13 fwd |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | -- |
| 备注: | 对于短暂的，细胞质表达的C-末端标记融合蛋白。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFLAG-CMV-5.1 4682 bp ds-DNA circular SYN 23-OCT-2018
DEFINITION Vector for expression of C-terminally FLAG-tagged proteins in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pFLAG-CMV-5.1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4682)
AUTHORS MilliporeSigma
TITLE Direct Submission
JOURNAL Exported Wednesday, March 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4682
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 318..697
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 924..990
/note="MCS"
/note="multiple cloning site"
CDS 993..1016
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
polyA_signal 1023..1645
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
rep_origin 1854..1989
/note="SV40 ori"
/note="SV40 origin of replication"
rep_origin complement(2476..3064)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3235..4095)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4096..4200)
/gene="bla"
/note="AmpR promoter"
rep_origin 4227..4682
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
901 tcgtttagtg aaccgtcaga attaagcttg cggccgcgaa ttcatcgata gatctgatat
961 cggtaccagt cgactctaga ggatcccggg ctgattacaa ggacgacgat gacaagtagg
1021 ttgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
1081 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
1141 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
1201 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
1261 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
1321 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
1381 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
1441 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
1501 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
1561 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
1621 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc
1681 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
1741 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
1801 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
1861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
1921 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
1981 ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt
2041 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
2101 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
2161 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
2221 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
2281 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
2881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4201 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
4261 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
4321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4441 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
4561 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
4621 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
4681 tt
//
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